Scientific program

The techniques performed at the course as well as the accompanying lectures will be grouped into four themes as shown below:

Part I. Production of ROS, RNS species and their fates

Introduction to oxidative stress
Production and biological role of ROS in biological systems
Production and biological role of RNS in biological systems.
Generating oxidative stress in experiments (ROS, RNS donors)

Part II Studying the biological effects of ROS/RNS in cells
Signaling roles of ROS/RNS
Lipid peroxidation
Protein oxidation
Protein nitration
DNA damage (oxidation and single strand breakage)

Part III Repair of oxidative damage
repair glycosylases
poly(ADP-ribosyl)ation (PAR-ylation)

Part IV Studying oxidative stress in vivo (lectures only)
ROS/RNS and diseases
PARylation and diseases

These themes will be broken down into daily program as shown below. Each day has it unique theme (e.g. ROS detection or DNA damage detection) and for each theme 3 different techniques (A,B,C menu) will be offered to the participants. Students can choose according to their interest. Participants will be grouped into 6 groups of 3. Each menu will be completed by two groups. In the afternoon, results will be shared and discussed between the groups in a Discussion section. (Students’ interest towards the techniques offered will be polled during the registration process allowing organizers to slightly adjust grouping of students according to their interests in different techniques.)

The core of the program is formed by 5 lab blocks (with morning and afternoon sections) which are accompanied by a limited number of lectures. The aim of most lectures (usually between 8:00-9:00) is to introduce the relevant methodologies, whereas a few lectures will aim at placing the techniques into context for participants who are less familiar with the theoretical background of these phenomena. For day 5, we chose rapid methods allowing all guests to enjoy a social program in the afternoon and evening amidst a busy schedule. Sufficient time will be allowed for discussion of results and for exchanging results/data between groups. This way students learn even more techniques than the number they perform.
Participating young scientists will be asked to present their work (student presentations). The best student presentation will be honoured by a small gift (according to lecturers vote).

Detailed Program (August 27-September 2, 2010)